Assembly of Minicellulosomes on the Surface of Bacillus subtilis †
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چکیده
Assembly of Minicellulosomes on the Surface of Bacillus subtilis † Timothy D. Anderson, Scott A. Robson,‡ Xiao Wen Jiang, G. Reza Malmirchegini, Henri-Pierre Fierobe, Beth A. Lazazzera, and Robert T. Clubb* Department of Chemistry and Biochemistry, UCLA-DOE Institute for Genomics and Proteomics, Molecular Biology Institute, and Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, 611 Charles E. Young Drive, Los Angeles, California 90095-1570, and Laboratoire de Chimie Bactérienne, IFR88-CNRS Marseille, France
منابع مشابه
Retraction for Anderson et al., "Assembly of Minicellulosomes on the Surface of Bacillus subtilis".
To cost-efficiently produce biofuels, new methods are needed to convert lignocellulosic biomass into fermentable sugars. One promising approach is to degrade biomass using cellulosomes, which are surface-displayed multicellulase-containing complexes present in cellulolytic Clostridium and Ruminococcus species. In this study we created cellulolytic strains of Bacillus subtilis that display one o...
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Two genes encoding EngB endoglucanase and mini-CbpA1 scaffolding protein of Clostridium cellulovorans were constructed and coexpressed in Bacillus subtilis WB800. The resulting minicellulosomes were isolated by gel filtration chromatography and characterized. Biochemical and immunological evidence indicated that fraction II contained minicellulosomes consisting of mini-CbpA1 and EngB. The in vi...
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Renewable lignocellulosic plant biomass is a promising feedstock from which to produce biofuels, chemicals, and materials. One approach to cost-effectively exploit this resource is to use consolidating bioprocessing (CBP) microbes that directly convert lignocellulose into valuable end products. Because many promising CBP-enabling microbes are non-cellulolytic, recent work has sought to engineer...
متن کاملSynthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation.
The ability of two strains of bacteria to cooperate in the synthesis of an enzyme complex (a minicellulosome) was examined. Three strains of Bacillus subtilis were constructed to express Clostridium cellulovorans genes engB, xynB, and minicbpA. MiniCbpA, EngB, and XynB were synthesized and secreted into the medium by B. subtilis. When the strains with the minicbpA and engB genes or with xynB we...
متن کاملSurface Decontamination by Dielectric Barrier Discharge Plasma
Background: Dielectric barrier discharge (DBD), a source of non-thermal plasma, is used in surface decontamination. Objective: To study the effect of DBD plasma treatment, we evaluated the effect of plasma exposure time on inactivation of Bacillus subtilis. Results: Applying the DBD plasma to the culture of B. subtilis caused complete sterilization of the surface without any thermal effects. In...
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تاریخ انتشار 2011